Most of the animals recovered fully from the procedure.

"Prior to an experiment the lizard was lightly anesthetized and immobilized by intramuscular injection of nembutal (0.01 mg á gA1) and tubocurarine chloride (Sigma; 0.03 mg á gA1). The lizard was positioned on its side, and respired artificially with a small rodent respirator through a small glass tube positioned at the tip of the trachea. The lid of the upward facing eye was held open with a drop of cyanoacrylate adhesive, and a thin film of 2% xylocaine gel (Astra) was spread on the surface of the cornea. ERGs were re- corded dfferentially with a pair of 0.13-mm platinum wires. The tip of the wire forming the active electrode was bent slightly and gently placed ̄at against the cornea just above the pupil. The wire forming the indfferent electrode was placed in a small slit made in the neck after application of xylocaine. At the end of the experi- ment the eye was rinsed with distilled water, the electrodes re- moved, and the eyelid gently pulled shut. The lizard remained on the respirator until all anesthesia effects had worn off. Most of the animals recovered fully from the procedure."

From The visual ecology of Puerto Rican anoline lizards: habitat light and spectral sensitivity by L.J. Fleishman á M. Bowman á D. Saunders W.E. Miller á M.J. Rury á E.R. Loew
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